Research use onlyFor laboratory and research purposes only — not for human consumption, medical, veterinary or diagnostic use.

PeptideStacks

Peptide reconstitution calculator

Convert a lyophilised peptide vial into a precise per-dose volume. Enter the vial size, the volume of bacteriostatic water you'll reconstitute with, and the research dose you need — the tool returns concentration in mg/mL and the corresponding insulin-syringe units.

Reconstitution calculator

Result

Concentration
2.500 mg/mL (2500 µg/mL)
Volume per dose
0.100 mL
Insulin syringe units
10.0 units

For laboratory and research use only. Reconstitution values are calculated arithmetic — not a recommendation for human administration. Verify concentrations against the original vial Certificate of Analysis.

How the arithmetic works

Reconstitution is straightforward arithmetic — concentration = mass ÷ volume. The complication for research handling is that lyophilised peptides come in milligrams, target doses are usually micrograms, and most subcutaneous research uses 1 mL insulin syringes graduated in 100 units rather than millilitres.

The standard formula:

concentration (µg/mL) = vial (mg) × 1000 / diluent (mL) volume per dose (mL) = target dose (µg) / concentration (µg/mL) insulin syringe units = volume (mL) × 100

Once you've reconstituted, store the solution at 2–8 °C. Most peptide solutions are stable for around 30 days at refrigerator temperature; longer storage requires aliquoting before freezing to avoid freeze-thaw degradation.

Worked example: 5 mg BPC-157 vial

Reconstitute 5 mg of BPC-157 with 2 mL of bacteriostatic water:

  • Concentration: 5 ÷ 2 = 2.5 mg/mL (2,500 µg/mL)
  • Target research dose: 250 µg
  • Volume per dose: 250 ÷ 2,500 = 0.1 mL
  • Insulin syringe units: 0.1 × 100 = 10 units

For laboratory research use only. The calculator is arithmetic — it does not constitute clinical advice and cannot account for individual research-subject variation. See our full research disclaimer.